The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
FunctionActin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia.
Tissue specificityUbiquitously expressed (at protein level).
Sequence similaritiesBelongs to the actin-binding proteins ADF family. Twinfilin subfamily. Contains 2 ADF-H domains.
Post-translational modificationsIn vitro, phosphorylated by PRKCZ, CK2 and SRC.