Overview

  • Product nameAnti-Twinkle antibody
    See all Twinkle primary antibodies
  • Description
    Rabbit polyclonal to Twinkle
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (GVFRKFATDN NCHVTLVIHP RKEDDDKELQ TASIFGSAKA SQEADNVLIL) of human Twinkle (NP_068602).

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration. Titer in peptide based assay is 1:312500.

Target

  • FunctionInvolved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
  • Tissue specificityHigh relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43.
  • Involvement in diseaseProgressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 3
    Sensory ataxic neuropathy dysarthria and ophthalmoparesis
    Mitochondrial DNA depletion syndrome 7
  • Sequence similaritiesContains 1 SF4 helicase domain.
  • Cellular localizationMitochondrion matrix > mitochondrion nucleoid. Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ataxin 8 antibody
    • Ataxin8 antibody
    • ATXN 8 antibody
    • ATXN8 antibody
    • C10 orf2 antibody
    • C10orf 2 antibody
    • C10orf2 antibody
    • Chromosome 10 open reading frame 2 antibody
    • IOSCA antibody
    • mitochondrial antibody
    • MTDPS7 antibody
    • PEO 1 antibody
    • PEO antibody
    • PEO1 antibody
    • PEO1_HUMAN antibody
    • PEOA3 antibody
    • Progressive external ophthalmoplegia 1 protein antibody
    • SANDO antibody
    • SCA 8 antibody
    • SCA8 antibody
    • T7 gp4 like protein with intramitochondrial nucleoid localization antibody
    • T7 gp4-like protein with intramitochondrial nucleoid localization antibody
    • T7 helicase-related protein with intramitochondrial nucleoid localization antibody
    • T7 like mitochondrial DNA helicase antibody
    • T7-like mitochondrial DNA helicase antibody
    • Twinkle protein antibody
    • Twinkle protein, mitochondrial antibody
    • TWINL antibody
    see all

Anti-Twinkle antibody images



  • Predicted band size : 77 kDa
    Western blot analysis of HeLa cell lysate labeling Twinkle with ab83329 at 1.0µg/ml.
  • Anti-Twinkle antibody (ab83329) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.
    12% gel concentration
  • ICC/IF image of ab83329 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83329, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Twinkle antibody (ab83329)

ab83329 has not yet been referenced specifically in any publications.

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The human protein has 3 isoforms: 77, 66, and 60 kDa (http://www.uniprot.org/uniprot/Q96RR1).
Thus, the band you are seeing around 57 kDa could be the third isoform at about 60 kDa. Please keep in mind that ...

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