Overview

  • Product nameAnti-Twist antibodySee all Twist primary antibodies ...
  • Description
    Rabbit polyclonal to Twist
  • Tested applicationsEMSA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Chimpanzee
  • Immunogen

    Synthetic peptide:

    PADDSLSNSEEEPDRQ

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 12-27 of Human Twist

  • Positive control
    • Osteosarcoma MG-63 cells. Human melanoma Mel15 cells. 293T cells expressing recombinant human Twist1.

Properties

Applications

Our Abpromise guarantee covers the use of ab50581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 21931630
IHC-P 1/500.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • FunctionActs as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificitySubset of mesodermal cells.
  • Involvement in diseaseDefects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS1 antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • TWIST antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • Twist1 antibody
    • TWST1_HUMAN antibody
    see all

Anti-Twist antibody images

  • Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
  • ab50581 staining Twist in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid buffer pH 6.1. Samples were then blocked with 0.5% BSA for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/500 dilution for 2 hours. A biotin-conjugated secondary antibody was used at a 1/100 dilution.
  • ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

References for Anti-Twist antibody (ab50581)

This product has been referenced in:
  • Shi Y  et al. Expression of the epithelial-mesenchymal transition-related proteins and their clinical significance in lung adenocarcinoma. Diagn Pathol 8:89 (2013). IHC-P ; Human . Read more (PubMed: 23706092) »
  • Whiteland H  et al. Putative prognostic epithelial-to-mesenchymal transition biomarkers for aggressive prostate cancer. Exp Mol Pathol 95:220-6 (2013). IHC-P ; Human . Read more (PubMed: 23933194) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (PAE cells)
Loading amount 75 µg
Specification PAE cells
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 25 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (293T - wild typoe andTwist transfected cells)
Loading amount 40 µg
Specification 293T - wild typoe andTwist transfected cells
Gel Running Conditions Reduced Denaturing (15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Vielen Dank für Ihre Antwort.

Ich werde Ihnen gerne nun die drei Ersatzröhrchen senden. Soll ich diese an die Warenannahme senden oder wollen Sie mir Ihre direkte Adresse senden? Ich brauche auch eine Telefonnummer für den Lief...

Read More

Vielen Dank für das Senden des ausgefüllten Fragebogens.

Leider sind mir ein paar Punkte noch nicht klar. Da es sich um drei Röhrchen handelt, möchte ich auch sicher gehen, dass wir nichts übersehen. Ich bitte Sie dah...

Read More

Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit dem neuen Lot von diesemAntikörper haben.

Ich habe den besprochenenFragebogen als Word-Dokument an diese E-Mail angehängt. Ich bin Ih...

Read More

Thank you for providing the image.

unfortunately, all expression databases I checked (EST Profile and BioGPS) do not show any expression for twist in liver or fibroblasts.

I therefore strongly recommend to run a positive control wit...

Read More

Thank you for contacting us.

ab50581is ananti-Twist, not anti-BCRP/ABCG2 antibody. Could you please confirm this with your customer?

Unfortunately we do not have a recommended protocol for EMSA.This application was added to the data...

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab50581. To check the status of the order please contact our Customer...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate cancer)
Specification Prostate cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"