Overview

  • Product name
  • Description
    Rabbit polyclonal to Twist
  • Tested applications
    Suitable for: EMSA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Chimpanzee
  • Immunogen

    Synthetic peptide:

    PADDSLSNSEEEPDRQ

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 12-27 of Human Twist

  • Positive control
    • Osteosarcoma MG-63 cells. Human melanoma Mel15 cells. 293T cells expressing recombinant human Twist1.

Properties

Applications

Our Abpromise guarantee covers the use of ab50581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 21931630
IHC-P 1/500.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).
ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

  • Function
    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificity
    Subset of mesodermal cells.
  • Involvement in disease
    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Anti-Twist antibody images

  • Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
  • ab50581 staining Twist in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid buffer pH 6.1. Samples were then blocked with 0.5% BSA for 30 minutes at room temperature followed by incubation with the primary antibody at a 1/500 dilution for 2 hours. A biotin-conjugated secondary antibody was used at a 1/100 dilution.
  • ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

References for Anti-Twist antibody (ab50581)

This product has been referenced in:
  • Song W  et al. ERß1 inhibits metastasis of androgen receptor-positive triple-negative breast cancer by suppressing ZEB1. J Exp Clin Cancer Res 36:75 (2017). Read more (PubMed: 28583190) »
  • Zhang L  et al. Dysregulation of Fra1 expression by Wnt/ß-catenin signalling promotes glioma aggressiveness through epithelial-mesenchymal transition. Biosci Rep 37:N/A (2017). Read more (PubMed: 28232512) »

See all 34 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human non-small cell lung carcinoma cell line)
Total protein in input
30 µg
Immuno-precipitation step
Protein A
Specification
human non-small cell lung carcinoma cell line
Username

Sara Sessa

Verified customer

Submitted Oct 21 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (A549)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
A549
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2016

Application
Western blot
Sample
Pig Cell lysate - whole cell (PAE cells)
Loading amount
75 µg
Specification
PAE cells
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 25 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (293T - wild typoe andTwist transfected cells)
Loading amount
40 µg
Specification
293T - wild typoe andTwist transfected cells
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2012

Vielen Dank für Ihre Antwort.

Ich werde Ihnen gerne nun die drei Ersatzröhrchen senden. Soll ich diese an die Warenannahme senden oder wollen Sie mir Ihre direkte Adresse senden? Ich brauche auch eine Telefonnummer für den Lief...

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Vielen Dank für das Senden des ausgefüllten Fragebogens.

Leider sind mir ein paar Punkte noch nicht klar. Da es sich um drei Röhrchen handelt, möchte ich auch sicher gehen, dass wir nichts übersehen. Ich bitte Sie dah...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit dem neuen Lot von diesemAntikörper haben.

Ich habe den besprochenenFragebogen als Word-Dokument an diese E-Mail angehängt. Ich bin Ih...

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Thank you for providing the image.

unfortunately, all expression databases I checked (EST Profile and BioGPS) do not show any expression for twist in liver or fibroblasts.

I therefore strongly recommend to run a positive control wit...

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Thank you for contacting us.

ab50581is ananti-Twist, not anti-BCRP/ABCG2 antibody. Could you please confirm this with your customer?

Unfortunately we do not have a recommended protocol for EMSA.This application was added to the data...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab50581. To check the status of the order please contact our Customer...

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1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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