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Recombinant fragment (Human)
Our Abpromise guarantee covers the use of ab50887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/50. Predicted molecular weight: 21 kDa.
Abcam recommends using BSA blocking.
|Dot blot||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
This image is courtesy of an Abreview submitted by Pawel MazurAll lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody
ChIP analysis using ab50887 binding Twist in Human primary macrophages. Cells were cross-linked for 15 minutes with formaldehyde. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. Protein binding was detected using real-time PCR.