Anti-Twist (phospho S68) antibody [EP18178] (ab187008)

Overview

  • Product name
    Anti-Twist (phospho S68) antibody [EP18178]
    See all Twist primary antibodies
  • Description
    Rabbit monoclonal [EP18178] to Twist (phospho S68)
  • Tested applications
    Suitable for: WB, IP, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Twist aa 50-150 (phospho S68). The exact sequence is proprietary.
    Database link: Q15672

  • Positive control
    • WB: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate. IP: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab187008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 21 kDa).
IP 1/60.
Dot blot 1/1000.

Target

  • Function
    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • Tissue specificity
    Subset of mesodermal cells.
  • Involvement in disease
    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Images

  • Dot blot analysis of Twist (phospho S68) labeled with ab187008 at 1/1000 dilution.

    Lane 1: Twist (phospho S68) peptide;

    Lane 2: Twist non-phospho peptide.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-Twist (phospho S68) antibody [EP18178] (ab187008) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 21 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

    PMID: 21502402.

  • Twist (phospho S68) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate with ab187008 at 1/60 dilution.

    Western blot was performed from the immunoprecipitate using ab187008 at 1/5000 dilution.

    VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate 10ug (Input).

    Lane 2: ab187008 IP in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187008 in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab187008 has not yet been referenced specifically in any publications.

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