Anti-Two pore calcium channel protein 2 antibody (ab119915)

Overview

  • Product nameAnti-Two pore calcium channel protein 2 antibody
    See all Two pore calcium channel protein 2 primary antibodies
  • Description
    Rabbit polyclonal to Two pore calcium channel protein 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the N-terminus of Human Two pore calcium channel protein 2 (NM_139075.3).

  • Positive control
    • MCF7 cell lysate, Human fetal small intestine tissue.

Properties

  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab119915 is purified by a peptide affinity column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab119915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/500.

Target

  • FunctionNicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.
  • Tissue specificityWidely expressed. Expressed at high level in liver and kidney.
  • Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
  • DomainEach of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationLysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP.
  • Information by UniProt
  • Database links
  • Alternative names
    • SHEP10 antibody
    • TPC2 antibody
    • TPC2_HUMAN antibody
    • tpcn2 antibody
    • Two pore calcium channel protein 2 antibody
    • two pore segment channel 2 antibody
    • Two-pore channel 2 antibody
    • Voltage dependent calcium channel protein TPC2 antibody
    • Voltage-dependent calcium channel protein TPC2 antibody
    see all

Anti-Two pore calcium channel protein 2 antibody images

  • Anti-Two pore calcium channel protein 2 antibody (ab119915) at 1/500 dilution + MCF7 cell lysate

    Predicted band size : 85 kDa

    The amount of protein per lane: 30ug

    PVDF membrane

    Blocking solution: 5% (W/V) skim milk, with 1 X TBST buffer

    Shake membrane at room temperature with ab119915, 1/500, for 30 minutes, then transfer to 4°C overnight.
  • ab119915 at 1/100 dilution staining Two pore calcium channel protein 2 in Formalin-fixed, Paraffin-embedded Human fetal small intestine tissue by Immunohistochemistry.

References for Anti-Two pore calcium channel protein 2 antibody (ab119915)

ab119915 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"