Anti-TXK antibody - Aminoterminal end (ab37818)

Overview

• Product name
  Anti-TXK antibody - Aminoterminal end
• Description
  Rabbit polyclonal to TXK - Aminoterminal end
• Specificity
  This antibody is specific for TXK.
• Tested applications
  Suitable for: WB, ELISA, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A KLH conjugated synthetic peptide selected from the sequence of human TXK within Ne terminal aa 1-100.

• Positive control
  • Breast carcinoma tissue and Jurkat cell lysate

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Purity
  Protein G purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Innate Immunity
  • Cytokines
  • Interferons

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Tec Family

  • Signal Transduction
  • Protein Phosphorylation
  • pTyr

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Positive Controls
  • Jurkat whole cell lysate (ab7899)

Applications

Target

• Function
  Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
• Tissue specificity
  Expressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential.
• Sequence similarities
  Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
  Contains 1 protein kinase domain.
  Contains 1 SH2 domain.
  Contains 1 SH3 domain.
• Post-translational
  modifications
  Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription.
  The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
• Cellular localization
  Cytoplasm. Nucleus. Cell membrane. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.

• Target information above from: UniProt accession P42681 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 7294 Human
  • Omim: 600058 Human
  • SwissProt: P42681 Human
  • Unigene: 479669 Human
• Alternative names
  • BTKL antibody
  • EC 2.7.10.2 antibody
  • MGC22473 antibody

  • Protein-tyrosine kinase 4 antibody
  • PSCTK5 antibody
  • PTK4 antibody
  • Resting lymphocyte kinase antibody
  • RLK antibody
  • TKL antibody
  • TXK antibody
  • TXK tyrosine kinase antibody
  • TXK_HUMAN antibody
  • Tyrosine protein kinase TXK antibody
  • Tyrosine-protein kinase TXK antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols