Overview

  • Product nameAnti-TXNDC3 antibody
    See all TXNDC3 primary antibodies
  • Description
    Mouse monoclonal to TXNDC3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: AEWRRLMGPT DPEEAKLLSP DSIRAQFGIS KLKNIVHGAS NAYEAKEVVN RLFEDPE, corresponding to amino acids 530-586 of Human TXNDC3 with tag (NP_057700).

  • Positive control
    • Jurkat cell lysate; Recombinant fragment of human TXNDC3.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevanceProbably required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of fibrous sheath (FS) proteins occurs. May be involved in the reduction of disulfide bonds within the sperm FS components. In vitro, it has neither NDP kinase nor reducing activity on disulfide bonds. TXNDC3 encodes a group II thioredoxin protein, composed of a thioredoxin domain and three NDP kinase domains, with testis specific expression.
  • Cellular localizationCytoplasm
  • Database links
  • Alternative names
    • CILD6 antibody
    • NM23 H8 antibody
    • NME8 antibody
    • Sperm specific thioredoxin 2 antibody
    • Spermatid specific thioredoxin 2 antibody
    • Sptrx 2 antibody
    • SPTRX2 antibody
    • Thioredoxin domain containing protein 3 antibody
    see all

Anti-TXNDC3 antibody images

  • Anti-TXNDC3 antibody (ab77658) at 5 µg/ml + Recombinant fragment of human TXNDC3 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Anti-TXNDC3 antibody (ab77658) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References for Anti-TXNDC3 antibody (ab77658)

ab77658 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"