Overview

  • Product nameAnti-TXNDC5 antibody
    See all TXNDC5 primary antibodies
  • Description
    Rabbit polyclonal to TXNDC5
  • Tested applicationsSuitable for: WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    His-tagged Fusion protein, corresponding to amino acids 76-417 of Mouse TXNDC5 (ERp46).

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
  • General notes


    ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Target

  • FunctionPossesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • EndoPDI antibody
    • Endoplasmic reticulum protein ERp46 antibody
    • Endoplasmic reticulum resident protein 46 antibody
    • Endothelial protein disulphide isomerase antibody
    • ER protein 46 antibody
    • ERp46 antibody
    • Hcc 2 antibody
    • MGC3178 antibody
    • PDIA15 antibody
    • Protein disulfide isomerase family A member 15 antibody
    • STRF8 antibody
    • Thioredoxin domain containing 5 (endoplasmic reticulum) antibody
    • Thioredoxin domain containing 5 antibody
    • Thioredoxin domain containing protein 5 antibody
    • Thioredoxin domain-containing protein 5 antibody
    • Thioredoxin like protein p46 antibody
    • Thioredoxin related protein antibody
    • Thioredoxin-like protein p46 antibody
    • TLP46 antibody
    • TXND5_HUMAN antibody
    • TXNDC 5 antibody
    • Txndc5 antibody
    see all

Anti-TXNDC5 antibody images

  • ICC/IF image of ab10292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10292 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-TXNDC5 antibody (ab10292) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 60 kDa,76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-TXNDC5 antibody (ab10292)

This product has been referenced in:
  • Alberti A  et al. ERp46 is reduced by high glucose and regulates insulin content in pancreatic beta-cells. Am J Physiol Endocrinol Metab 297:E812-21 (2009). WB, ICC/IF ; Mouse . Read more (PubMed: 19622788) »
  • Knoblach B  et al. ERp19 and ERp46, New Members of the Thioredoxin Family of Endoplasmic Reticulum Proteins. Mol Cell Proteomics 2:1104-19 (2003). Read more (PubMed: 12930873) »

See all 2 Publications for this product

Product Wall

Thanks for your enquiry. At the moment we don't carry any rabbit isotype controls (they are still new to our product range). You may want to try R&D Systems or Zymed, I know they both carry it. If you have any additional questions, please let us know.

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Thank you for your email. If you are interested in ordering multiple vials of the same antibody, I can offer you a discount. However, if you are interested in ordering one each of the 5 antibodies that you listed, I cannot offer you a discount. Shippin...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"