Overview

  • Product nameAnti-TXNDC5 antibody
    See all TXNDC5 primary antibodies
  • Description
    Goat polyclonal to TXNDC5
  • SpecificityThis antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: SLHRFVLSQAKDEL, corresponding to C terminal amino acids 347-360 of Human TXNDC5.

  • Positive control
    • Human Placenta Lysate
  • General notesGenBank Accession Number - NP_110437; NP_071368

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab13820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human TXNDC5 peptide (ab23242).
IHC-P Use at an assay dependent concentration. PubMed: 23326410
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionPossesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 3 thioredoxin domains.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • EndoPDI antibody
    • Endoplasmic reticulum protein ERp46 antibody
    • Endoplasmic reticulum resident protein 46 antibody
    • Endothelial protein disulphide isomerase antibody
    • ER protein 46 antibody
    • ERp46 antibody
    • Hcc 2 antibody
    • MGC3178 antibody
    • PDIA15 antibody
    • Protein disulfide isomerase family A member 15 antibody
    • STRF8 antibody
    • Thioredoxin domain containing 5 (endoplasmic reticulum) antibody
    • Thioredoxin domain containing 5 antibody
    • Thioredoxin domain containing protein 5 antibody
    • Thioredoxin domain-containing protein 5 antibody
    • Thioredoxin like protein p46 antibody
    • Thioredoxin related protein antibody
    • Thioredoxin-like protein p46 antibody
    • TLP46 antibody
    • TXND5_HUMAN antibody
    • TXNDC 5 antibody
    • Txndc5 antibody
    see all

Anti-TXNDC5 antibody images



  • Predicted band size : 50 kDa
    Ab13820 staining (0.1µg/ml) of Human Placenta lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence

References for Anti-TXNDC5 antibody (ab13820)

This product has been referenced in:
  • Wang L  et al. Investigate pathogenic mechanism of TXNDC5 in rheumatoid arthritis. PLoS One 8:e53301 (2013). WB, IHC-P ; Mouse . Read more (PubMed: 23326410) »
  • Pace PE  et al. Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. Biochem J 453:475-85 (2013). IP, WB ; Human . Read more (PubMed: 23713588) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Mouse Cell (cardiomyocyte)
Specification cardiomyocyte
Permeabilization Yes - 0.5% Tritonx-100
Fixative Formaldehyde
Username

Ms. shuang zhang

Verified customer

Submitted Dec 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"