Overview

  • Product name
  • Description
    Rabbit polyclonal to TXNIP
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 300-350 of Human TXNIP (NP_006463.3).

  • Positive control
    • HeLa whole cell lysate

Applications

Our Abpromise guarantee covers the use of ab114981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells.
    • Sequence similarities
      Belongs to the arrestin family.
    • Post-translational
      modifications
      Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EST01027 antibody
      • HHCPA78 antibody
      • THIF antibody
      • Thioredoxin binding protein 2 antibody
      • Thioredoxin interacting protein antibody
      • Thioredoxin-binding protein 2 antibody
      • Thioredoxin-interacting protein antibody
      • TXNIP antibody
      • TXNIP_HUMAN antibody
      • Upregulated by 1,25 dihydroxyvitamin D 3 antibody
      • VDUP1 antibody
      • Vitamin D3 up-regulated protein 1 antibody
      see all

    Images

    • Detection of TXNIP in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114981 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    This product has been referenced in:
    • Johnson ML  et al. Mechanism by Which Caloric Restriction Improves Insulin Sensitivity in Sedentary Obese Adults. Diabetes 65:74-84 (2016). Read more (PubMed: 26324180) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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