Overview

  • Product name
  • Description
    Rabbit polyclonal to TXNRD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 231-488 of Human TXNRD1 (Uniprot ID Q16881)

  • Positive control
    • MOLT4 whole cell lysate, mouse brain and rat brain lysate/extracts, paraformaldehyde-fixed HeLa cells, paraffin-embedded Cal27 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab155790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • Tissue specificity
    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • Sequence similarities
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • Domain
    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • Post-translational
    modifications
    The N-terminus of isoform 5 is blocked.
    ISGylated.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
    • Gene associated with retinoid IFN induced mortality 12 protein antibody
    • GRIM 12 antibody
    • GRIM-12 antibody
    • GRIM12 antibody
    • KDRF antibody
    • KM 102 derived reductase like factor antibody
    • KM-102-derived reductase-like factor antibody
    • MGC9145 antibody
    • Oxidoreductase antibody
    • Thioredoxin reductase 1 antibody
    • Thioredoxin reductase 1 cytoplasmic antibody
    • Thioredoxin reductase GRIM 12 antibody
    • Thioredoxin reductase TR1 antibody
    • TR 1 antibody
    • TR antibody
    • TR1 antibody
    • TRXR 1 antibody
    • TRXR1 antibody
    • TRXR1_HUMAN antibody
    • TXNR antibody
    • TXNRD 1 antibody
    • Txnrd1 antibody
    see all

Images

  • Anti-TXNRD1 antibody (ab155790) at 1/3000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 71 kDa



    7.5% SDS PAGE
  • Anti-TXNRD1 antibody (ab155790) at 1/1000 dilution + Mouse brain extract/lysate at 50 µg

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • Anti-TXNRD1 antibody (ab155790) at 1/1000 dilution + Rat brain lysate/extract at 50 µg

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling TRXR1 with ab155790 at 1/200 dilution (green). Bottom image shows merge with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling TRXR1 with ab155790 at 1/500 dilution.

References

ab155790 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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