Overview

  • Product nameAnti-TXNRD1 antibody
    See all TXNRD1 primary antibodies
  • Description
    Rabbit polyclonal to TXNRD1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Chimpanzee, Ferret, Macaque Monkey, Orangutan
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
IP
WB
  • Application notesICC/IF: Use at an assay dependent concentration.
    IHC-P: 1/400 (PMID 20064251).
    WB: 1/2000. Predicted molecular weight: 55 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIsoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
    • Tissue specificityIsoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
    • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
      Contains 1 glutaredoxin domain.
    • DomainThe N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
    • Post-translational
      modifications
      The N-terminus of isoform 5 is blocked.
      ISGylated.
    • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all

    Anti-TXNRD1 antibody images



    • Predicted band size : 55 kDa


      ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.

      Lane 1: HeLa cell lysates.

      Lane 2: 293T cell lysates.

      Lane 3: Jurkat cell lysates.

    • TXNRD1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TXNRD1 (ab16840) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16840.
      Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 55kDa: TXNRD1. Non specific band - 65kDa: We are unsure as to the identity of this extra band.
    • ab16840 at 1/100 dilution detecting TXNRD1 from human EA hy 926 cells by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected with an Alexa-Fluor 488® goat anti-rabbit IgG.

      This image is coutesy of an Abreview submitted by Adam Szadkowski on 5 January 2006.

      See Abreview

    • ab16840 staining TXNRD1 in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
      Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab16840 at 1/400 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent A and B in 1× PBS) was applied at 25°C for 30 minutes. After 3 washes with 1× PBS, staining was visualized with peroxidase-sensitive 3,3'-Diaminobenzidine tablets. Exposure times were synchronized so that all tissues samples within an antibody group were exposed to DAB for the exact same time. All slides were counterstained with 0.1% methyl green for 3 minutes at 60°C, dehydrated in ethanol, cleared in xylene and mounted with Permount. Images were obtained at 40× using a Leica DMI4000B confocal microscope with the Retiga 2000R digital camera. Exposure times were kept constant for all samples.

      Image shows TXNRD1 distribution in normal mammary tissue, benign hyperplasia tissue (BH), ductal carcinoma in site (DCIS) and invasive breast cancer tissue (IBC).
    • ab16840 (2µg/ml) staining TXNRD1 in human breast using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining of acini.
      Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    References for Anti-TXNRD1 antibody (ab16840)

    This product has been referenced in:
    • Chung PJ  et al. MicroRNA-205 targets tight junction-related proteins during urothelial cellular differentiation. Mol Cell Proteomics 13:2321-36 (2014). Read more (PubMed: 24912853) »
    • Ben-Lulu S  et al. A substrate trapping approach identifies proteins regulated by reversible S-nitrosylation. Mol Cell Proteomics 13:2573-83 (2014). Read more (PubMed: 24973421) »

    See all 13 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Chinese Hamster Cell lysate - whole cell (CHO)
    Specification CHO
    Treatment transfected
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Thank you for contacting us.

    As we discussed please use these antibodies without any hesitation, If these fails please let us know.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (EA hy 926)
    Specification EA hy 926
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
    Username

    Mr. Adam Szadkowski

    Verified customer

    Submitted Jan 05 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (EA hy926)
    Specification EA hy926
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Mr. Adam Szadkowski

    Verified customer

    Submitted Nov 10 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"