Overview

  • Product name
  • Description
    Rabbit polyclonal to TXNRD1
  • Specificity
    Thioredoxin reductase 1
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    PHRIKATNNKGKEK

    conjugated to KLH by a Cysteine residue linker, corresponding to amino acids 137-150 of Human TXNRD1

  • Positive control
    • Rat brain microsomal preparation HeLa cells U937 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab78629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
ICC 1/1000.

Target

  • Function
    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • Tissue specificity
    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • Sequence similarities
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • Domain
    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • Post-translational
    modifications
    The N-terminus of isoform 5 is blocked.
    ISGylated.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
    • Gene associated with retinoid IFN induced mortality 12 protein antibody
    • GRIM 12 antibody
    • GRIM-12 antibody
    • GRIM12 antibody
    • KDRF antibody
    • KM 102 derived reductase like factor antibody
    • KM-102-derived reductase-like factor antibody
    • MGC9145 antibody
    • Oxidoreductase antibody
    • Thioredoxin reductase 1 antibody
    • Thioredoxin reductase 1 cytoplasmic antibody
    • Thioredoxin reductase GRIM 12 antibody
    • Thioredoxin reductase TR1 antibody
    • TR 1 antibody
    • TR antibody
    • TR1 antibody
    • TRXR 1 antibody
    • TRXR1 antibody
    • TRXR1_HUMAN antibody
    • TXNR antibody
    • TXNRD 1 antibody
    • Txnrd1 antibody
    see all

Images

  • Anti-TXNRD1 antibody (ab78629) at 1 µg/ml + Rat brain microsomal preparation at 40 µg

    Secondary
    Goat anti rabbit IgG conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • U937 cells fixed in paraformaldehyde and permeabilized were labelled with ab78629 at 1/100 dilution followed by anti-rabbit-Alexa 488 secondary antibody conjugate at 1/100 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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