Overview

  • Product nameAnti-TXNRD1 antibody [EPNCIR129]
    See all TXNRD1 primary antibodies
  • Description
    Rabbit monoclonal [EPNCIR129] to TXNRD1
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse TXNRD1.
    Database link: Q9JMH6

  • Positive control
    • Mouse liver, NIH 3T3, RAW264.7, Human fetal liver and Rat liver lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-TXNRD1 antibody (Alexa Fluor® 488) [EPNCIR129] (ab203260)
    Anti-TXNRD1 antibody (Alexa Fluor® 647) [EPNCIR129] (ab203261)

    Anti-TXNRD1 antibody (HRP) [EPNCIR129] (ab203464)

Properties

Applications

Our Abpromise guarantee covers the use of ab124954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55, 67 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • FunctionIsoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • Tissue specificityIsoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • DomainThe N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • Post-translational
    modifications
    The N-terminus of isoform 5 is blocked.
    ISGylated.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
    • Gene associated with retinoid IFN induced mortality 12 protein antibody
    • GRIM 12 antibody
    • GRIM-12 antibody
    • GRIM12 antibody
    • KDRF antibody
    • KM 102 derived reductase like factor antibody
    • KM-102-derived reductase-like factor antibody
    • MGC9145 antibody
    • Oxidoreductase antibody
    • Thioredoxin reductase 1 antibody
    • Thioredoxin reductase 1 cytoplasmic antibody
    • Thioredoxin reductase GRIM 12 antibody
    • Thioredoxin reductase TR1 antibody
    • TR 1 antibody
    • TR antibody
    • TR1 antibody
    • TRXR 1 antibody
    • TRXR1 antibody
    • TRXR1_HUMAN antibody
    • TXNR antibody
    • TXNRD 1 antibody
    • Txnrd1 antibody
    see all

Anti-TXNRD1 antibody [EPNCIR129] images

  • All lanes : Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified)

    Lane 1 : rat liver lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 55, 67 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

    Predicted band size : 55, 67 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified) + HepG2 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

    Predicted band size : 55, 67 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HeLa cells with purified ab124954 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124954 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human testis with purified ab124954 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ab124954 (purified) at 1/40 immunoprecipitating TXNRD1 in 10 μg Jurkat (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124954 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
  • All lanes : Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse liver lysates
    Lane 2 : NIH 3T3 lysates
    Lane 3 : RAW264.7 lysates
    Lane 4 : Human fetal liver lysates
    Lane 5 : Rat liver lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 55, 67 kDa
  • Unpurified ab124954, at 1/100 dilution, staining TXNRD1 in HeLa cells by Immunofluorescence.

References for Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

This product has been referenced in:
  • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
  • Tobe R  et al. High error rates in selenocysteine insertion in mammalian cells treated with the antibiotic doxycycline, chloramphenicol, or geneticin. J Biol Chem 288:14709-15 (2013). Read more (PubMed: 23589299) »

See all 4 Publications for this product

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