Recombinant
RabMAb

Anti-TXNRD1 antibody [EPR10204(B)] (ab151716)

Overview

  • Product name
    Anti-TXNRD1 antibody [EPR10204(B)]
    See all TXNRD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10204(B)] to TXNRD1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human TXNRD1 (UniProt: Q16881).

  • Positive control
    • Jurkat, HeLa and A549 cell lysates; Human testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab151716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
    • Tissue specificity
      Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
    • Sequence similarities
      Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
      Contains 1 glutaredoxin domain.
    • Domain
      The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
    • Post-translational
      modifications
      The N-terminus of isoform 5 is blocked.
      ISGylated.
    • Cellular localization
      Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all

    Images

    • All lanes : Anti-TXNRD1 antibody [EPR10204(B)] (ab151716) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 71 kDa
    • Immunohistochemical analysis of Paraffin-embedded Human testis tissue labeling TXNRD1 with ab151716 at 1/50 dilution.

    References

    ab151716 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151716.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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