SpecificityAb16841 antibody is cross-reactive; it detects both TXNRD1 and TXNRD2. The antibody has been tested in western blotting in jurkat whole cell lysate, rat lung lysate, mouse liverlysate. In both rat and mouse lysates it detected two bands, the upper one being TXNRD2.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 56 kDa.
FunctionMaintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling.
Tissue specificityHighly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low.
Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
All lanes : Anti-TXNRD2 antibody (ab16841) at 1/2000 dilution
Lane 1 : Jurkat lysate Lane 2 : Mouse liver lysate Lane 3 : Rat lung lysate
Predicted band size : 56 kDa
In mouse and rat lysate the upper band is TXNRD2, we do not know what the lower non specific band is .
References for Anti-TXNRD2 antibody (ab16841)
This product has been referenced in:
Zhao X et al. Variations of thioredoxin system contributes to increased susceptibility to apoptosis in cardiomyocytes of type 2 diabetic rats. Acta Biochim Biophys Sin (Shanghai)46:318-29 (2014).
Read more (PubMed: 24578161) »