Overview

  • Product nameAnti-TXNRD2 antibody
    See all TXNRD2 primary antibodies
  • Description
    Rabbit polyclonal to TXNRD2
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • human breast carcinoma tissue (IHC) HT29 cell extract (WB)

Properties

Applications

Our Abpromise guarantee covers the use of ab58445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMaintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling.
  • Tissue specificityHighly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • Selenoprotein Z antibody
    • SelZ antibody
    • Thioredoxin reductase 2 antibody
    • Thioredoxin reductase 2 mitochondrial antibody
    • Thioredoxin reductase 3 antibody
    • Thioredoxin reductase beta antibody
    • Thioredoxin reductase TR3 antibody
    • TR 3 antibody
    • TR antibody
    • TR beta antibody
    • TR-beta antibody
    • TR3 antibody
    • TRXR 2 antibody
    • TRXR2 antibody
    • TRXR2_HUMAN antibody
    • TXNRD 2 antibody
    • Txnrd2 antibody
    see all

Anti-TXNRD2 antibody images

  • 1/50 ab58445 staining paraffin-embedded human breast carcinoma tissue by Immunohistochemistry with and without blocking peptide.
  • All lanes : Anti-TXNRD2 antibody (ab58445) at 1/500 dilution

    Lane 1 : extracts from HT29 cells
    Lane 2 : extracts from HT29 cells with blocking peptide


    Predicted band size : 56 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TXNRD2 antibody (ab58445) at 1/500 dilution

    Lane 1 : Mouse Penile Tissue (Sample 1)
    Lane 2 : Mouse Penile Tissue (Sample 2)

    Lysates/proteins at 25 µg per lane.

    Secondary
    An HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/4000 dilution

    Predicted band size : 56 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa (possible non-specific binding).

    This image is courtesy of an Abreview submitted by Dr. Gwen Iagoda

    See Abreview

  • IHC image of ab58445 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab58445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TXNRD2 antibody (ab58445)

This product has been referenced in:
  • Shearn CT  et al. Increased carbonylation of the lipid phosphatase PTEN contributes to Akt2 activation in a murine model of early alcohol-induced steatosis. Free Radic Biol Med 65:680-92 (2013). Mouse . Read more (PubMed: 23872024) »
  • Godoy JR  et al. Redox atlas of the mouse Immunohistochemical detection of glutaredoxin-, peroxiredoxin-, and thioredoxin-family proteins in various tissues of the laboratory mouse. Biochim Biophys Acta 1810:2-92 (2011). IHC ; Mouse . Read more (PubMed: 20682242) »

See all 3 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat Penile Tissue Section)
Specification Rat Penile Tissue Section
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization Yes - PBS + Triton (0.4%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Mar 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Penile Tissue)
Loading amount 25 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (10%, 18 well Criterion Gel)
Blocking step Starting Block T20 (TBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Apr 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Penile Tissue Homogenates)
Loading amount 60 µg
Specification Penile Tissue Homogenates
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Nov 03 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"