The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 134 kDa.
Use a concentration of 3 µg/ml.
Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
Involvement in disease
Defects in TYK2 are the cause of protein-tyrosine kinase 2 deficiency (TYK2 deficiency) [MIM:611521]; also known as autosomal recessive hyper-IgE syndrome (HIES) with atypical mycobacteriosis. TYK2 deficiency consists of a primary immunodeficiency characterized by recurrent skin abscesses, pneumonia, and highly elevated serum IgE.
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
The FERM domain mediates interaction with JAKMIP1.
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: TYK2 knockout HAP1 cell lysate (20 µg) Lane 3: Raji cell lysate (20 µg) Lane 4: MDA-MB-361 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab57678 observed at 135 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab57678 was shown to specifically react with TYK2 when TYK2 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab57678 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
IHC-P - TYK2 antibody (ab57678)
TYK2 antibody (ab57678) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma.
Western blot - TYK2 antibody (ab57678)
Predicted band size : 134 kDa TYK2 antibody (ab57678) at 1ug/lane + HeLa cell lysate at 25ug/lane.