Anti-TYRO3 antibody [OTI5B7] (ab117877)
Key features and details
- Mouse monoclonal [OTI5B7] to TYRO3
- Suitable for: Flow Cyt (Intra), IP, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-TYRO3 antibody [OTI5B7]
See all TYRO3 primary antibodies -
Description
Mouse monoclonal [OTI5B7] to TYRO3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human TYRO3. Protein expressed in HEK-293T cells.
Database link: Q06418 -
Positive control
- ICC/IF: pCMV6-ENTRY TYRO3 cDNA transfected COS-7 cells. Flow Cyt (Intra): HEK-293T cells transfected with overexpress plasmid. IP: DDK tagged TYRO3 overexpression lysates.
-
General notes
The clone number has been updated from 5B7 to OTI5B7, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading...
-
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI5B7 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IP |
Use a concentration of 4 µg/ml.
|
|
ICC/IF |
1/100.
|
Notes |
---|
Flow Cyt (Intra)
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use a concentration of 4 µg/ml. |
ICC/IF
1/100. |
Target
-
Function
May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Abundant in the brain and lower levels in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 7301 Human
- Omim: 600341 Human
- SwissProt: Q06418 Human
- Unigene: 381282 Human
-
Alternative names
- Brt antibody
- BYK antibody
- DTK antibody
see all
Images
-
pCMV6-ENTRY TYRO3 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for TYRO3 using ab117877 at a 1/100 dilution in ICC/IF.
-
For each experiment, 500 µl of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK-293T lysate), 2 µg of ab117877 and 20 µl (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Lane 1 : Negative control: IP without adding ab117877
Lane 2 : Anti-TYRO3 antibody [OTI5B7] (ab117877) at 2 µg
All lanes : -
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY TYRO3 (Red) or empty vector control plasmid (Blue) were immunostained with ab117877 at 1/100 dilution and then analyzed by Flow Cytometry (Intracellular).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab117877 has not yet been referenced specifically in any publications.