Recombinant
RabMAb

Anti-Tyrosinase antibody [EPR10141] (ab170905)

Overview

  • Product name
    Anti-Tyrosinase antibody [EPR10141]
    See all Tyrosinase primary antibodies
  • Description
    Rabbit monoclonal [EPR10141] to Tyrosinase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Tyrosinase. The exact sequence is proprietary.
    Database link: P14679

  • Positive control
    • Human melanoma lysate, Human melanoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
    • Involvement in disease
      Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
      Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
    • Sequence similarities
      Belongs to the tyrosinase family.
    • Cellular localization
      Melanosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    Images

    • ab170905 staining Tyrosinase in A375 (human malignant melanoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Control: PBS only.

    • Flow cytometry analysis of A375 (human malignant melanoma) cells labeling with purified ab170905 at 1/30 dilution ( 10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) (1/2000 dilution) was used as the secondary antibody. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

       

       

    • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue, labeling Tyrosinase using ab170905 at a 1/100 dilution.

    • Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution + Human melanoma lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 60 kDa

    References

    This product has been referenced in:
    • Huang YC  et al. Fenofibrate suppresses melanogenesis in B16-F10 melanoma cells via activation of the p38 mitogen-activated protein kinase pathway. Chem Biol Interact 205:157-64 (2013). WB . Read more (PubMed: 23872139) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse melanoma (B16F10), human melanoma (SKMel-3),)
    Gel Running Conditions
    Reduced Denaturing (Criterion TGX 4- 15% (BioRad)))
    Loading amount
    40 µg
    Specification
    mouse melanoma (B16F10), human melanoma (SKMel-3),
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Username

    Mercedes Quintana

    Verified customer

    Submitted Sep 30 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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