Overview

  • Product nameAnti-Tyrosinase antibody [T311]
    See all Tyrosinase primary antibodies
  • Description
    Mouse monoclonal [T311] to Tyrosinase
  • SpecificityStudies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein.

  • Positive control
    • Melanoma

Properties

Applications

Our Abpromise guarantee covers the use of ab738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.
WB Use at an assay dependent concentration.

Target

  • FunctionThis is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • Involvement in diseaseDefects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • Sequence similaritiesBelongs to the tyrosinase family.
  • Cellular localizationMelanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATN antibody
    • CMM8 antibody
    • LB24 AB antibody
    • LB24-AB antibody
    • Monophenol monooxygenase antibody
    • OCA1 antibody
    • OCA1A antibody
    • OCAIA antibody
    • Oculocutaneous albinism IA antibody
    • SHEP3 antibody
    • SK29 AB antibody
    • SK29-AB antibody
    • Tumor rejection antigen AB antibody
    • TYR antibody
    • TYRO_HUMAN antibody
    • tyrosinase (oculocutaneous albinism IA) antibody
    • Tyrosinase antibody
    see all

Anti-Tyrosinase antibody [T311] images

  • Anti-Tyrosinase antibody [T311] (ab738) at 1/250 dilution + Human A375 whole cell lysate at 10000 cells

    Secondary
    HRP-conjugated Horse anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab738 staining Tyrosinase in human neonatal foreskin (NHM) cells by Immunocytochemistry/ Immunofluorescence.
    NHM grown on coverslips were fixed and probed with anti-NCKX5-(I) (green) and ab738 (red). Merged images are shown.

References for Anti-Tyrosinase antibody [T311] (ab738)

This product has been referenced in:
  • Gutiérrez-Juárez G  et al. Optical Photoacoustic Detection of Circulating Melanoma Cells In Vitro. Int J Thermophys 31:784-792 (2010). ICC/IF ; Human . Read more (PubMed: 20730036) »
  • Ginger RS  et al. SLC24A5 Encodes a trans-Golgi Network Protein with Potassium-dependent Sodium-Calcium Exchange Activity That Regulates Human Epidermal Melanogenesis. J Biol Chem 283:5486-95 (2008). ICC/IF ; Human . Read more (PubMed: 18166528) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us.

The IgG concentration of lot GR33380 is 66.7ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount 10000 cells
Specification A375 - melanoma cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Username

Mr. Rudolf Jung

Verified customer

Submitted Sep 06 2011

Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab97...

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Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"