Anti-Tyrosinase antibody [T311] (Biotin) (ab79673)

Overview

  • Product nameAnti-Tyrosinase antibody [T311] (Biotin)
    See all Tyrosinase primary antibodies
  • Description
    Mouse monoclonal [T311] to Tyrosinase (Biotin)
  • ConjugationBiotin
  • SpecificityShows no cross-reaction with MAGE1 and tyrosinase-related protein 1, TRP1/gp75.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Tyrosinase protein.

  • Positive control
    • Melanoma cell lines and melanoma tissue.
  • General notes


    Clone T311 is a useful marker for melanocytes and melanomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab79673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.

Use at a concentration of 1 - 2 µg/ml for 2hrs at RT.

IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use at a concentration of 2 - 4 µg/ml for 30min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionThis is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • Involvement in diseaseDefects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • Sequence similaritiesBelongs to the tyrosinase family.
  • Cellular localizationMelanosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATN antibody
    • CMM8 antibody
    • LB24 AB antibody
    • LB24-AB antibody
    • Monophenol monooxygenase antibody
    • OCA1 antibody
    • OCA1A antibody
    • OCAIA antibody
    • Oculocutaneous albinism IA antibody
    • SHEP3 antibody
    • SK29 AB antibody
    • SK29-AB antibody
    • Tumor rejection antigen AB antibody
    • TYR antibody
    • TYRO_HUMAN antibody
    • tyrosinase (oculocutaneous albinism IA) antibody
    • Tyrosinase antibody
    see all

References for Anti-Tyrosinase antibody [T311] (Biotin) (ab79673)

This product has been referenced in:
  • Chen YT  et al. Immunophenotyping of melanomas for tyrosinase: implications for vaccine development. Proc Natl Acad Sci U S A 92:8125-9 (1995). WB, ICC/IF ; Human . Read more (PubMed: 7667256) »

See 1 Publication for this product

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Thank you for your enquiry and for you interest in ab79673.

Although we do not know of any references illustrating use of this antibody to stain mouse tissue via IHC-P, we do have information that it has been tested to successfully react with...

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Thank you for your reply. The code *** can be used for testing ab54447 in IHC-Fr. This code will expire on August 30th, 2012. I hope this helps, please let me know if you have any additional questions or concerns.

DISCOUNT CODE: ***

Expiration date: August 12th, 2012

I am very pleased to hear you would like to accept our offer and test ab79673 in IHC-Fr. This code will give you 1 free primary antibody before the expiration date. To redeem th...

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