Overview

  • Product name
    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to Tyrosine Hydroxylase
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, IHC-FrFl, ICC/IF, IHC-FoFr, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Cat, Human, Pig
    Predicted to work with: Mammal
  • Immunogen

    Full length SDS denatured protein (purified from pheochromocytoma) (Rat).

  • General notes

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/750 - 1/1500.
IHC-P 1/750.
IHC-FrFl 1/1000. PubMed: 222726
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20357108
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
ICC 1/2000.

Target

  • Function
    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity
    Mainly expressed in the brain and adrenal glands.
  • Pathway
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Images

  • Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (panel 1). Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution

    Lane 1 : Mouse cell lysate (CATH.a neuron)
    Lane 2 : Mouse cell lysate (CATH.a neuron)
    Lane 3 : Mouse cell lysate (CATH.a neuron)
    Lane 4 : Mouse cell lysate (CATH.a neuron)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat Anti-Rabbit HRP
    Developed using the ECL technique

    Predicted band size : 60 kDa


    Exposure time : 50 seconds

    Image courtesy of an anonymous AbReview

  • ab112 staining tyrosine hydroxulase in mouse brain tissue. Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.

  • ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody. The image of Caudate putamen shows characteristic nerve fibre positivity

    See Abreview



  • Predicted band size : 60 kDa

    Western Blot: ab112 - WB analysis of Tyrosine Hydroxylase in PC12 cell whole lysate.

  • PFA-fixed, Triton X-100 permeabilized human neurons in cerebral organoid stained for Tyrosine Hydroxylase (green) using ab112 at 1/300 dilutionl in ICC/IF.

  • ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry. The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

    See Abreview



  • Predicted band size : 60 kDa

    Western Blot: ab112 - 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.

  • ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

    See Abreview

  • ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections). Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.

    See Abreview

  • Immunohistochemical staining of retina tissue using ab112.

References

This product has been referenced in:
  • Longo F  et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-a-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun 5:22 (2017). IHC-FrFl ; Mouse . Read more (PubMed: 28292328) »
  • Chen G  et al. Antidyskinetic Effects of MEK Inhibitor Are Associated with Multiple Neurochemical Alterations in the Striatum of Hemiparkinsonian Rats. Front Neurosci 11:112 (2017). IHC-P, ICC/IF ; Rat . Read more (PubMed: 28337120) »

See all 56 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Brain
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neurons in human cerebral organoid)
Permeabilization
Yes - Triton X-100
Specification
neurons in human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Radhika Menon

Verified customer

Submitted Jul 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Nerve surrounding common hepatic artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Nerve surrounding common hepatic artery
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Mr. Tamas Bellak

Verified customer

Submitted Aug 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 18 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (heart)
Specification
heart
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (CATH.a neuron)
Specification
CATH.a neuron
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 30 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample
Mouse Cultured Cells (CATH.a neuron)
Specification
CATH.a neuron
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 06 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 10 2014

1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up