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Full length SDS denatured protein (purified from pheochromocytoma) (Rat).
ab112 can be used as a marker for dopaminergic and noradrenergic neurons.
Our Abpromise guarantee covers the use of ab112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/750 - 1/1500.|
|IHC-FrFl||1/1000. PubMed: 222726|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20357108|
|WB||1/200. Predicted molecular weight: 60 kDa.|
|IP||Use at an assay dependent concentration.|
ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody. The image of Caudate putamen shows characteristic nerve fibre positivity
Western Blot: ab112 - WB analysis of Tyrosine Hydroxylase in PC12 cell whole lysate.
ab112 staining tyrosine hydroxulase in mouse brain tissue. Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.
ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry. The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
Western Blot: ab112 - 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.
ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections). Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.
Immunohistochemical staining of retina tissue using ab112.
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