The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/750 - 1/1500.
1/1000. PubMed: 222726
Use at an assay dependent concentration. PubMed: 20357108
1/200. Predicted molecular weight: 60 kDa.
Use at an assay dependent concentration.
FunctionPlays an important role in the physiology of adrenergic neurons.
Tissue specificityMainly expressed in the brain and adrenal glands.
PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab112)This image is courtesy of an Abreview submitted by Carl Hobbs
ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody. The image of Caudate putamen shows characteristic nerve fibre positivity
ab112 staining tyrosine hydroxulase in mouse brain tissue. Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an anonymous Abreview
ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry. The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
Western blot - Anti-Tyrosine Hydroxylase antibody (ab112)
Predicted band size : 60 kDa
Western Blot: ab112 - 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus
ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.
Immunohistochemistry (Frozen sections) - Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Xiaobin Liu.
ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections). Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.
Immunohistochemical staining of retina tissue using ab112.
References for Anti-Tyrosine Hydroxylase antibody (ab112)
This product has been referenced in:
Longo F et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-a-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun5:22 (2017).
Read more (PubMed: 28292328) »
Chen G et al. Antidyskinetic Effects of MEK Inhibitor Are Associated with Multiple Neurochemical Alterations in the Striatum of Hemiparkinsonian Rats. Front Neurosci11:112 (2017).
Read more (PubMed: 28337120) »