Overview

  • Product name
    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description
    Sheep polyclonal to Tyrosine Hydroxylase
  • Tested applications
    Suitable for: IHC-FrFl, ICC/IF, IHC-Fr, WB, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Mammal
  • Immunogen

    Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
    Database link: P04177

  • General notes

    ab113 can be used as a marker for dopaminergic and noradrenergic neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl 1/1000 - 1/2000.
ICC/IF 1/1000.
IHC-Fr 1/1000.
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Plays an important role in the physiology of adrenergic neurons.
  • Tissue specificity
    Mainly expressed in the brain and adrenal glands.
  • Pathway
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in disease
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similarities
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody images

  • Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

  • ab113 staining Tyrosine Hydroxylase in murine brain tissue by Immunohistochemistry (Free Floating).Tissue was incubated with ab113 at a 1/1000 dilution for 48 hours at 4°C. The secondary used was a Dylight 594 conjugated donkey anti-sheep IgG (H+L) (ab96941) used at a 1/500 dilution.

    See Abreview

  • ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.

    This image is courtesy of an Abreview submitted by Jim Deuchars.

    See Abreview



  • Predicted band size : 60 kDa

    Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.

References for Anti-Tyrosine Hydroxylase antibody (ab113)

This product has been referenced in:
  • Yokoyama T  et al. Quantification of sympathetic hyperinnervation and denervation after myocardial infarction by three-dimensional assessment of the cardiac sympathetic network in cleared transparent murine hearts. PLoS One 12:e0182072 (2017). Read more (PubMed: 28753665) »
  • Borges G  et al. Activation of Extracellular Signal-Regulated Kinases (ERK 1/2) in the Locus Coeruleus Contributes to Pain-Related Anxiety in Arthritic Male Rats. Int J Neuropsychopharmacol 20:463 (2017). IHC-FrFl ; Rat . Read more (PubMed: 28158734) »

See all 24 Publications for this product

Product Wall

Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab113.
To check th...

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Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain slice)
Specification
brain slice
Username

Dr. Xiaobin Liu

Verified customer

Submitted Jul 18 2011

Thank you for your patience. I have received confirmation from the originator of the product and it seems that there is no specific data on whether the antibody recognizes all isoforms of histone H1, as we do not currently have access to the antigen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain)
Specification
brain
Fixative
Paraformaldehyde
Username

Prof. Jim Deuchars

Verified customer

Submitted May 16 2006

Dear Sir: Both of these antibodies can be used for immunocytochemistry and immunohistochemistry. The difference is that the NB300-108 is sensitive enough to stain fibers in the cerebral cortex. The cost of these two antibodies is $175. We wou...

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The antibody should work for this application. For a reference, see: Lewis, D.A., et al. Four Isoforms of Tyrosine Hydroxylase are Expressed in Human Brain. 54(2): 477-492, 1993.

raised against native rat - purified from pheochromocytoma, host species is sheep, can be used to detect all mammalian forms,WB gives a band at ~60 kDa [1:200 dilution (S35-protein A)], can be used in IP (1:10), IH, and IC (1:2000),supplied in 15 ug un...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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