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Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase. SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma (rat)
Database link: P04177
ab113 can be used as a marker for dopaminergic and noradrenergic neurons.
Our Abpromise guarantee covers the use of ab113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||1/1000 - 1/2000.|
|WB||1/200. Predicted molecular weight: 60 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.
ab113 at 1/2000 dilution staining rat brain cells (ab29475) by immunocyctochemistry. The antibody was incubated with the tissue for 48 hours and then bound antibody was detected using a biotinylated donkey anti-sheep antibody. The image shows the staining of free-floating 50 micron brainstem sections, labelling A5cell groups. The antibody also labels fine varicosities of axons.
This image is courtesy of an Abreview submitted by Jim Deuchars.
Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.