Anti-Tyrosine Hydroxylase antibody (ab117112)

Overview

  • Product nameAnti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to Tyrosine Hydroxylase
  • Tested applicationsSuitable for: IHC-FoFr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Dog, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Tyrosine Hydroxylase.

  • Positive control
    • This antibody gave a positive signal in FFPE rat brain 6 week sagittal tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab117112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/3000.
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPlays an important role in the physiology of adrenergic neurons.
  • Tissue specificityMainly expressed in the brain and adrenal glands.
  • PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody images

  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab117112) at 1/10000 dilution

    Lane 1 : Mouse Brain Tissue lysate(striatum)
    Lane 2 : Mouse Brain Tissue lysate(cortex)
    Lane 3 : Mouse Brain Tissue lysate(substantia nigra)
    Lane 4 : Mouse Brain Tissue lysate(hippocampus)

    Lysates/proteins at 30 µg per lane.

    Secondary
    Donkey anti-rabbit HRP cojugated polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 58 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Karine Thibault

    See Abreview

  • IHC-FoFr image of Tyrosine 3-monooxygenase staining in ventral tegmental area of the mouse cortex. Tissue was fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using cryostat. Samples were incubated with ab117112 (1/3000) for 18 hours at 25°C and were secondary stained with AlexaFluor® 488 conjugated donkey anti-rabbit antibody (1/1000).

    See Abreview

  • IHC image of Tyrosine 3-monooxygenase staining in sagittal 6 week rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117112, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Tyrosine Hydroxylase antibody (ab117112)

ab117112 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (gel 10% SDS-PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Karine Thibault

Verified customer

Submitted Nov 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Karine Thibault

Verified customer

Submitted Aug 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"