Anti-Tyrosine Hydroxylase antibody (ab51191)

Overview

  • Product nameAnti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description
    Rabbit polyclonal to Tyrosine Hydroxylase
  • SpecificityThis antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
  • Tested applicationsSuitable for: IHC-FoFr, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from Tyrosine Hydroxylase around the phosphorylation site of Serine 19 (AVSPEQ) (Human)

  • Positive control
    • Extracts from 3T3 cells treated with Tyrosine Hydroxylase antibody; Human brain tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20923695
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 59 kDa).

Target

  • FunctionPlays an important role in the physiology of adrenergic neurons.
  • Tissue specificityMainly expressed in the brain and adrenal glands.
  • PathwayCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • Involvement in diseaseDefects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51191 (1/50 dilution). Left: without immunizing peptide, Right: with immunizing peptide
  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab51191) at 1/300 dilution

    Lane 1 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with no peptide
    Lane 2 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with peptide


    Predicted band size : 59 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

References for Anti-Tyrosine Hydroxylase antibody (ab51191)

This product has been referenced in:
  • Chung K  et al. Structural and molecular interrogation of intact biological systems. Nature 497:332-7 (2013). Read more (PubMed: 23575631) »
  • Cambon K  et al. Aggressive behavior during social interaction in mice is controlled by the modulation of tyrosine hydroxylase expression in the prefrontal cortex. Neuroscience 171:840-51 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20923695) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

The blocking peptide for ab51191 has been published as Tyrosine Hydroxylase peptide (ab151686):

http://www.abcam.com/tyrosine-hydroxylase-peptide-ab151686.html



Please let me know ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Apr 16 2012

Thank you for your inquiry.
We do guarantee both of these antibodies, ab51191 and ab112, to work on rat IHC-P. Therefore both should be adapted for your study. The ab112 has been used up to 1/750 in IHC-P and has been produced against the full len...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"