The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Use at an assay dependent concentration.
Plays an important role in the physiology of adrenergic neurons.
Mainly expressed in the brain and adrenal glands.
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Immunohistochemical analysis of paraffin-embedded human brain (left apical lobe) tissue using ab59276 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
Western blot - Tyrosine Hydroxylase antibody (ab59276)
All lanes : Anti-Tyrosine Hydroxylase antibody (ab59276) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells with no immunising peptide Lane 2 : Extracts from COLO205 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 59 kDa Observed band size : 59 kDa