Anti-Tyrosine Hydroxylase antibody (ab76442)

Overview

  • Product name
    Anti-Tyrosine Hydroxylase antibody
    See all Tyrosine Hydroxylase primary antibodies
  • Description
    Chicken polyclonal to Tyrosine Hydroxylase
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-FoFr, IHC-FrFl, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.

Properties

Applications

Our Abpromise guarantee covers the use of ab76442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000. using 2% paraformaldehyde-fixed tissues or cells.
IHC-P 1/1000 - 1/2000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20653037
IHC-FrFl 1/1000.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Plays an important role in the physiology of adrenergic neurons.
    • Tissue specificity
      Mainly expressed in the brain and adrenal glands.
    • Pathway
      Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
    • Involvement in disease
      Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
      Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
    • Sequence similarities
      Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    • Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).

    • ab76442 staining Tyrosine Hydroxylase in murine brain tissue by Immunohistochemistry (Frozen sections).

      Tissue was fixed with paraformaldehyde, permeabilizd by Triton X-100 and blocked with 3% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour at 37°C. ab6753 HRP-conjugated rabbit anti-chicken polyclonal IgG (ab6753) (1/500) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Morales I  et al. The astrocytic response to the dopaminergic denervation of the striatum. J Neurochem 139:81-95 (2016). Read more (PubMed: 27230040) »
    • Schmitt D  et al. Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons. Histochem Cell Biol 146:489-512 (2016). ICC/IF . Read more (PubMed: 27344443) »

    See all 20 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - Triton X100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 15 2015

    Vielen Dank für Ihre Anfrage.
    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 90.000 Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0,1%
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 06 2012

    Thank you for your inquiry.

    I can confirm that the authors of this publication (Mousa et al., 2011) acquired the testinginformation from theproducing labof this antibody directly.

    I suggest thereforeto cite this publication for the ...

    Read More


    Les 2 anticorps anti-Tyrosine Hydroxylaseab76442 et ab53417 (retiré du catalogue) sont tous les 2 des IgY dirigés contre les mêmes peptides immunogènes.Cependant nous ne pouvons dire que ce sont exactement les même...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain, 20 µm frozen section)
    Specification
    brain, 20 µm frozen section
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Username

    Dorothee Domenger

    Verified customer

    Submitted Jul 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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