Anti-Tyrosine Hydroxylase antibody (ab6211)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit polyclonal to Tyrosine Hydroxylase -
Host species
Rabbit -
Specificity
Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme. -
Tested applications
Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Chicken, Chimpanzee, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
Database link: P04177 -
General notes
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration.
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WB |
1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
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ICC/IF |
1/1000.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. |
WB
1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa. |
ICC/IF
1/1000. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Plays an important role in the physiology of adrenergic neurons. -
Tissue specificity
Mainly expressed in the brain and adrenal glands. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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Database links
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 1292 Mouse
- Unigene: 11082 Rat
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Alternative names
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
Images
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ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg
Performed under reducing conditions.
Predicted band size: 42,53,84 kDaThree bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.
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ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
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Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
Datasheets and documents
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Datasheet download
References (66)
ab6211 has been referenced in 66 publications.
- Solié C et al. Superior Colliculus to VTA pathway controls orienting response and influences social interaction in mice. Nat Commun 13:817 (2022). PubMed: 35145124
- Na J et al. Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones. Int J Mol Sci 23:N/A (2022). PubMed: 35269751
- Han X et al. Neuronal SH2B1 attenuates apoptosis in an MPTP mouse model of Parkinson's disease via promoting PLIN4 degradation. Redox Biol 52:102308 (2022). PubMed: 35390677
- Montardy Q et al. Dopamine modulates visual threat processing in the superior colliculus via D2 receptors. iScience 25:104388 (2022). PubMed: 35633939
- Celada L et al. Differential HIF2α Protein Expression in Human Carotid Body and Adrenal Medulla under Physiologic and Tumorigenic Conditions. Cancers (Basel) 14:N/A (2022). PubMed: 35740651