Anti-Tyrosyl tRNA synthetase/TyrRS antibody (ab155504)
Key features and details
- Rabbit polyclonal to Tyrosyl tRNA synthetase/TyrRS
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Tyrosyl tRNA synthetase/TyrRS antibody
See all Tyrosyl tRNA synthetase/TyrRS primary antibodies -
Description
Rabbit polyclonal to Tyrosyl tRNA synthetase/TyrRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Tyrosyl tRNA synthetase/TyrRS aa 307-528.
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Positive control
- 293T, A431, H1299, HeLaS3, MOLT4, Raji whole cell lysates; A431 cells
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General notes
Keep as a concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). -
Involvement in disease
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 tRNA-binding domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281581 Cow
- Entrez Gene: 8565 Human
- Entrez Gene: 107271 Mouse
- Entrez Gene: 313047 Rat
- Omim: 603623 Human
- SwissProt: Q29465 Cow
- SwissProt: P54577 Human
- SwissProt: Q91WQ3 Mouse
see all -
Alternative names
- CMTDIC antibody
- SYYC_HUMAN antibody
- Tyrosine tRNA ligase, cytoplasmic antibody
see all
Images
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Anti-Tyrosyl tRNA synthetase/TyrRS antibody (ab155504) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed A431 cells labeling Tyrosyl tRNA synthetase / TyrRS with ab155504 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155504 has not yet been referenced specifically in any publications.