Anti-Tyrosyl tRNA synthetase antibody (ab31535)

Overview

  • Product nameAnti-Tyrosyl tRNA synthetase antibody
    See all Tyrosyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Tyrosyl tRNA synthetase
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • HEK293 for WB, SH-SY-5Y for IF.

Properties

Applications

Our Abpromise guarantee covers the use of ab31535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/3000 - 1/10000.
WB 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 58 kDa).
IP 1/3000 - 1/10000.

Target

  • FunctionCatalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • Involvement in diseaseDefects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Anti-Tyrosyl tRNA synthetase antibody images

  • Anti-Tyrosyl tRNA synthetase antibody (ab31535) at 1/3000 dilution + HEK293 cell lysate at 30 µg

    Predicted band size : 58 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of SH-SY-5Y cells with ab31535 at 1/3000.
  • Anti-Tyrosyl tRNA synthetase antibody (ab31535) at 1/10000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 58 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab31535 staining Tyrosyl tRNA synthetase in human SH-SY-5Y cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Tyrosyl tRNA synthetase antibody (ab31535)

ab31535 has not yet been referenced specifically in any publications.

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