Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961)


  • Product name
    Anti-Tyrosyl tRNA synthetase antibody [YAR5H08]
    See all Tyrosyl tRNA synthetase primary antibodies
  • Description
    Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase
  • Tested applications
    Suitable for: WB, IP, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human YARS fragment

  • Positive control
    • HeLa cells and cell lysate and NIH3T3 cell lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab50961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 20 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.


  • Function
    Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • Involvement in disease
    Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities
    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] images

  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

References for Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961)

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »

See 1 Publication for this product

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Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

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