The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionPeroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and the specific processing of PTS1 proteins. May participate to a regulatory mechanism that control the peroxisomal beta-oxidation of fatty acids.
Sequence similaritiesBelongs to the peptidase S1B family.
Post-translational modificationsProcessed into the 49 kDa and the 10 kDa forms upon import into the peroxisomes. The 49 kDa form probably corresponds to the mature enzyme, while the 10 kDa form may represent an inhibitory N-terminal fragment which prevents protease activity until it reaches the peroxisomes.