Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: 1/8000.
    WB: Use at a concentration of 2 - 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
    • Tissue specificity
      Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
    • Sequence similarities
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 1 BTB (POZ) domain.
      Contains 3 C2H2-type zinc fingers.
    • Domain
      The C-terminal zinc finger domain functions as a transcriptional transactivator.
      The BTB (POZ) domain possesses repressor activity.
    • Cellular localization
      Nucleus. Located in nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • FANCC interacting protein antibody
      • FANCC-interacting protein antibody
      • Fanconi anemia zinc finger protein antibody
      • FAXF antibody
      • FAZF antibody
      • OTTHUMP00000045790 antibody
      • repressor of GATA antibody
      • Rog antibody
      • Testis zinc finger protein antibody
      • ZBT32_HUMAN antibody
      • Zbtb32 antibody
      • ZBTB32 protein antibody
      • Zinc finger and BTB domain containing 32 antibody
      • Zinc finger and BTB domain-containing protein 32 antibody
      • Zinc finger protein 538 antibody
      • ZNF538 antibody
      see all

    Images

    • Anti-TZFP antibody (ab26066) at 2 µg/ml + Human Bone Marrow lysate (35µg protein in RIPA buffer)

      Predicted band size : 53 kDa

    References

    ab26066 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab26066.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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