The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: 1/8000.
WB: Use at a concentration of 2 - 5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
Tissue specificityPredominantly expressed in testis. Some isoforms are ubiquitously expressed.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 3 C2H2-type zinc fingers.
DomainThe C-terminal zinc finger domain functions as a transcriptional transactivator. The BTB (POZ) domain possesses repressor activity.
Cellular localizationNucleus. Located in nuclear speckles.