The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:12500.
FunctionDNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
Tissue specificityPredominantly expressed in testis. Some isoforms are ubiquitously expressed.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 3 C2H2-type zinc fingers.
DomainThe C-terminal zinc finger domain functions as a transcriptional transactivator. The BTB (POZ) domain possesses repressor activity.
Cellular localizationNucleus. Located in nuclear speckles.