Overview

  • Product nameAnti-TZFP antibody
    See all TZFP primary antibodies
  • Description
    Rabbit polyclonal to TZFP
  • SpecificityThis antibody recognizes TZFP
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    A region within synthetic peptide: PIRLPSPYGS DRLVQLAARL RPALCDTLIT VGSQEFPAHS LVLAGVSQQL, corresponding to amino acids 5-54 of Human TZFP

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

Target

  • FunctionDNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • Tissue specificityPredominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • DomainThe C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • Cellular localizationNucleus. Located in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Anti-TZFP antibody images

  • Anti-TZFP antibody (ab49349) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa

References for Anti-TZFP antibody (ab49349)

ab49349 has not yet been referenced specifically in any publications.

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