Overview

  • Product name
  • Description
    Mouse polyclonal to TZFP
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human TZFP with proprietary tag, amino acids 1-302 (AAH17700.1)

  • Positive control
    • Human colon tissue lysate, TZFP transfected 293T cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • Tissue specificity
    Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • Domain
    The C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • Cellular localization
    Nucleus. Located in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Images

  • Anti-TZFP antibody (ab88594) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • All lanes : Anti-TZFP antibody (ab88594) at 5 µg/ml

    Lane 1 : TZFP transfected 293t cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg/ml per lane.


    Predicted band size : 53 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ab88594 at 10µg/ml staining TZFP in HeLa cells by Immunofluorescence.

References

ab88594 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (transfected 293T cells)
Specification
transfected 293T cells
Total protein in input
2e+006 cells
Username

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Verified customer

Submitted Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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