Overview

  • Product nameAnti-TZFP antibody
    See all TZFP primary antibodies
  • Description
    Mouse polyclonal to TZFP
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human TZFP with proprietary tag, amino acids 1-302 (AAH17700.1)

  • Positive control
    • Human colon tissue lysate, TZFP transfected 293T cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionDNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • Tissue specificityPredominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • DomainThe C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • Cellular localizationNucleus. Located in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Anti-TZFP antibody images

  • Anti-TZFP antibody (ab88594) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • All lanes : Anti-TZFP antibody (ab88594) at 5 µg/ml

    Lane 1 : TZFP transfected 293t cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg/ml per lane.


    Predicted band size : 53 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ab88594 at 10µg/ml staining TZFP in HeLa cells by Immunofluorescence.

References for Anti-TZFP antibody (ab88594)

ab88594 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (transfected 293T cells)
Specification transfected 293T cells
Total protein in input 2e+006 cells
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Submitted Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"