U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14903)

Overview

  • Product nameU-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundGlioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14903)

    ab14903 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    Unfortunately, I am sorry to confirm we do not sell U-87 MG whole cell lysate, only the nuclear lysate (ab14903).

    I would like to recommend checking the Biocompare website which has an excellent antibod...

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    Thank you for your interest in ab14903. This lysate is a protein lysate only and is not suitable for DNA/RNA work. Unfortunately we do not have cell lines or tissues available for RNA isolation on our catalog. I apologize for any inconvenience t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"