Overview

  • Product nameU0126
  • Description
    Selective MKK inhibitor
  • Biological descriptionSelective non-competitive inhibitor of MAP kinase kinase (MKK). Inhibits MKK1, MKK2 (IC50 values of 0.07 and 0.06 μM, respectively) and MKK5 with little or no effect on a wide range of other kinases. Centrally active following systemic administration in vivo.
  • Purity> 98%

Properties

    U0126 images

    • Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

      Lane 1: Marker
      Lane 2: A431 lysate starved
      Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
      Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
      Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

      Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

      Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

      Observed bands:
      RSK1p90 (pS380) band size: 83 kDa
      AKT1 (pS473) band size: 56 kDa
      ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
      RPS6 (pS235/236) band size: 31 kDa
      Rab11a band size: 24 kDa
      Unknown band: 130kDa

    References for U0126 (ab120241)

    This product has been referenced in:
    • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
    • Favata MF  et al. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem 273:18623-32 (1998). Read more (PubMed: 9660836) »
    • Duncia JV  et al. MEK inhibitors: the chemistry and biological activity of U0126, its analogs, and cyclization products. Bioorg Med Chem Lett 8:2839-44 (1998). Read more (PubMed: 9873633) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"