• Product nameAnti-U1A antibody
    See all U1A primary antibodies
  • Description
    Mouse monoclonal to U1A
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-283 of Human U1A


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab55751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionBinds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
  • Sequence similaritiesBelongs to the RRM U1 A/B'' family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • fc19d01 antibody
    • Mud1 antibody
    • small nuclear ribonucleoprotein polypeptide A antibody
    • snRNP A antibody
    • snRNP protein A antibody
    • SNRPA antibody
    • SNRPA_HUMAN antibody
    • U1 small nuclear ribonucleoprotein A antibody
    • U1 small nuclear RNP specific A antibody
    • U1 snRNP A antibody
    • U1 snRNP specific protein A antibody
    • U1-A antibody
    • U1A antibody
    • wu:fc19d01 antibody
    • zgc:77810 antibody
    see all

Anti-U1A antibody images

  • U1A antibody (ab55751) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart tissue.

  • Predicted band size : 31 kDa
    U1A antibody (ab55751) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55751 at 10 ug/ml staining U1A in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55751, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-U1A antibody (ab55751)

This product has been referenced in:
  • Ammon T  et al. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol 6:312-23 (2014). Human . Read more (PubMed: 24872507) »
  • Hodson MJ  et al. The transition in spliceosome assembly from complex E to complex A purges surplus U1 snRNPs from alternative splice sites. Nucleic Acids Res : (2012). WB . Read more (PubMed: 22505580) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Sample Mouse Cell lysate - whole cell (NSC34 cells)
Specification NSC34 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Cheng Song

Verified customer

Submitted Aug 26 2013