Overview

  • Product name
  • Description
    Mouse monoclonal to U1A
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-283 of Human U1A

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab166890 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
  • Sequence similarities
    Belongs to the RRM U1 A/B'' family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • fc19d01 antibody
    • Mud1 antibody
    • small nuclear ribonucleoprotein polypeptide A antibody
    • snRNP A antibody
    • snRNP protein A antibody
    • SNRPA antibody
    • SNRPA_HUMAN antibody
    • U1 small nuclear ribonucleoprotein A antibody
    • U1 small nuclear RNP specific A antibody
    • U1 snRNP A antibody
    • U1 snRNP specific protein A antibody
    • U1-A antibody
    • U1A antibody
    • wu:fc19d01 antibody
    • zgc:77810 antibody
    see all

Images

  • U1A antibody (ab55751) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart tissue.


  • Predicted band size : 31 kDa
    U1A antibody (ab55751) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55751 at 10 ug/ml staining U1A in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55751, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Llorian M  et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . Read more (PubMed: 27317697) »
  • Rogalska ME  et al. Therapeutic activity of modified U1 core spliceosomal particles. Nat Commun 7:11168 (2016). Read more (PubMed: 27041075) »

See all 5 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Sample
Mouse Cell lysate - whole cell (NSC34 cells)
Specification
NSC34 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Cheng Song

Verified customer

Submitted Aug 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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