Overview

  • Product name
  • Description
    Rabbit polyclonal to U1SNRNPBP
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A region within synthetic peptide: VWRAMLARYV PNKGVIGDPL LTLFVARLNL QTKEDKLKEV FSRYGDIRRL, corresponding to N terminal amino acids 36-85 of Human U1SNRNPBP

  • Positive control
    • Jurkat cell lysate, Human kidney

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

Titre using peptide based assay 1/1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    U1SNRNPBP is a homolog of U1-snRNP binding protein. Its N-terminal contains an RNA recognition motif. The C-terminal is rich in Arg/Asp and Arg/Glu dipeptides, commonly found in splicing factors. Exists in at least two isoforms produced by alternative splicing that differ in the 5' sequence regions.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • HM 1 antibody
    • Small nuclear ribonucleoprotein U11/U12 subunit 35 antibody
    • SNRNP35 antibody
    • U1 snRNP binding protein homolog antibody
    • U11/U12 snRNP 35K antibody
    • U11/U12 snRNP 35K, isoform b antibody
    • U11/U12 snRNP antibody
    see all

Anti-U1SNRNPBP antibody images

  • Anti-U1SNRNPBP antibody (ab51035) at 2.5 µg/ml + Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab51035 at 4.0 µg/ml staining epithelial cells of renal tubules from human kidney; paraffin embedded; magnification 400x.

References for Anti-U1SNRNPBP antibody (ab51035)

This product has been referenced in:
  • Shen H  et al. The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing. Genes Dev 24:2389-94 (2010). WB ; Human . Read more (PubMed: 21041408) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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