The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: antibody detection limit dilution 1/32000
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 26 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.
Tissue specificityIsoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes.
Sequence similaritiesBelongs to the splicing factor SR family. Contains 2 C3H1-type zinc fingers. Contains 1 RRM (RNA recognition motif) domain.
DomainThe second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA splicing events.