Overview

  • Product nameAnti-U2AF35 antibody
    See all U2AF35 primary antibodies
  • Description
    Rabbit polyclonal to U2AF35
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
  • Immunogen

    A region between residue 140 and 190 of Human U2AF35 (NP_006749.1).

  • Positive control
    • Human: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Lung Adenocarcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma and Stomach Adenocarcinoma tissue Mouse: Colon Carcinoma CT26 and Renal Cell Carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab100805 is affinity purified using an epitope specific to U2AF35 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab100805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • FunctionPlays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
  • Sequence similaritiesBelongs to the splicing factor SR family.
    Contains 2 C3H1-type zinc fingers.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp313J1712 antibody
    • FP793 antibody
    • Human U2 snRNP auxiliary factor small subunit9 antibody
    • RN antibody
    • RNU2AF1 antibody
    • Splicing factor U2AF 35 kDa subunit antibody
    • U2 auxiliary factor 35 kDa subunit antibody
    • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
    • U2 small nuclear RNA auxiliary factor 1 antibody
    • U2 snRNP auxiliary factor small subunit antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
    • U2AF1 antibody
    • U2AF1_HUMAN antibody
    • U2AF35 antibody
    • U2AFBP antibody
    see all

Anti-U2AF35 antibody images

  • ab100805, at a 1/250 dilution, staining U2AF35 in a formalin-fixed, paraffin-embedded section of Human stomach carcinoma by Immunohistochemistry. Detection by DAB staining.
  • ab100805, at a 1/250 dilution, staining U2AF35 in a formalin-fixed, paraffin-embedded section of mouse colon carcinoma by Immunohistochemistry. Detection by DAB staining.

References for Anti-U2AF35 antibody (ab100805)

ab100805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"