• Product name
  • Description
    Rabbit polyclonal to U2AF35
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 140 and 190 of human U2AF35 (NP_006749.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86305 was affinity purified using an epitope specific to U2AF35 immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab86305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 2 C3H1-type zinc fingers.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp313J1712 antibody
    • FP793 antibody
    • Human U2 snRNP auxiliary factor small subunit9 antibody
    • RN antibody
    • RNU2AF1 antibody
    • Splicing factor U2AF 35 kDa subunit antibody
    • U2 auxiliary factor 35 kDa subunit antibody
    • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
    • U2 small nuclear RNA auxiliary factor 1 antibody
    • U2 snRNP auxiliary factor small subunit antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
    • U2AF1 antibody
    • U2AF1_HUMAN antibody
    • U2AF35 antibody
    • U2AFBP antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling U2AF35 with ab86305 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-U2AF35 antibody (ab86305) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa
    Observed band size: 36 kDa (why is the actual band size different from the predicted?)

    Exposure time: 3 minutes
  • Detection of U2AF35 by Western Blot of Immunprecipitate.
    ab86305, at 1 µg/ml, staining U2AF35 in HeLa whole cell lysate immunoprecipitated using ab86305 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 10 seconds.


This product has been referenced in:
  • Martinez NM  et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev 29:2054-66 (2015). Read more (PubMed: 26443849) »
  • Zhang J  et al. Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinities. Proc Natl Acad Sci U S A 112:E4726-34 (2015). WB . Read more (PubMed: 26261309) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Human Fibroblast)
Loading amount
20 µg
Human Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jan 11 2013


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