U87 MG nuclear extract lysate (ab14903)

Overview

  • Product nameU87 MG nuclear extract lysate
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundGlioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

References for U87 MG nuclear extract lysate (ab14903)

ab14903 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Unfortunately, I am sorry to confirm we do not sell U-87 MG whole cell lysate, only the nuclear lysate (ab14903).

I would like to recommend checking the Biocompare website which has an excellent antibod...

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Thank you for your interest in ab14903. This lysate is a protein lysate only and is not suitable for DNA/RNA work. Unfortunately we do not have cell lines or tissues available for RNA isolation on our catalog. I apologize for any inconvenience t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"