U937 cytoplasmic extract lysate (ab14904)

Overview

  • Product name
    U937 cytoplasmic extract lysate
    See all U937 lysates
  • General notes
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

References

ab14904 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. U937 cells grow in suspension. To make them adhere, you can try using poly-lysine, fibronectin or collagen coated plates. I hope this information helps, please do not hesitate to contact us if you need any more advice...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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