U937 nuclear extract lysate (IFN alpha treated) (ab14907)

Overview

  • Product name
    U937 nuclear extract lysate (IFN alpha treated)
    See all U937 lysates
  • Description
    U937 nuclear extract lysate
  • General notes
    Cells were cultured in medium supplemented with 5000 U/mL interferon alpha for one hour at 37°C immediately prior to harvesting. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

References

ab14907 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your phone call the other day. I have heard back from the production lab regarding the IFN alpha treatment of the cell prior to lysis, and I received the following response: "The treatment with IFN alpha has no effect on the stabili...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up