General notesCells were cultured in medium supplemented with 5 nM TPA (phorbol,
12-myristate, 13 acetate) for 72 hours at 37°C immediately prior to
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
BackgroundThe U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.
References for U937 nuclear extract lysate (TPA stimulated) (ab14910)
has not yet been referenced specifically in any publications.
Publishing research using ab14910? Please let us know so that we can cite the reference in this datasheet.
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