Overview
- Product nameuPA Human ELISA kitSee all Urokinase type Plasminogen Activator kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 5 pg/ml
- Range62.5 pg/ml - 4000 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119611 Urokinase type Plasminogen Activator (uPA) Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Urokinase type Plasminogen Activator specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Urokinase type Plasminogen Activator amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-human Urokinase type Plasminogen Activator antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-human Urokinase type Plasminogen Activator antibody 1 x 130µl Lyophilized recombinant human Urokinase type Plasminogen Activator standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml - Research Areas
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
Target information above from: UniProt accession
P00749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATFBDPLT5Plasminogen activator urinary
- PLAUQPDu PAU-plasminogen activatoruPAURKUROK_HUMANUrokinase-type plasminogen activator chain B
see all
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Applications
Our Abpromise guarantee covers the use of ab119611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
uPA Human ELISA kit images
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Sandwich ELISA - Urokinase type Plasminogen Activator Human ELISA Kit (ab119611)Representative standard curve using ab119611.
Protocols
References for uPA Human ELISA kit (ab119611)
ab119611 has not yet been referenced specifically in any publications.
