Overview

  • Product nameAnti-UACA antibody
    See all UACA primary antibodies
  • Description
    Rabbit polyclonal to UACA
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to amino acids 675-725 (KAKLAQHVKP EEHEQVKSRL EQKSGELGKK ITELTLKNQT LQKEIEKVYL D) of human UACA (NP_060473.2). (Peptide available as ab134313.)

  • Positive control
    • Human controls: Anaplastic thyroid carcinoma, breast carcinoma, colon carcinoma, Linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, stomach adenocarcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab84478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500.

Target

  • FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
    Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
  • Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
  • Sequence similaritiesContains 6 ANK repeats.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ10128 antibody
    • KIAA1561 antibody
    • MGC141967 antibody
    • MGC141969 antibody
    • Nuclear membrane binding protein antibody
    • NUCLING antibody
    • UACA antibody
    • UACA_HUMAN antibody
    • Uveal autoantigen with coiled coil domains and ankyrin repeats antibody
    • Uveal autoantigen with coiled-coil domains and ankyrin repeats antibody
    see all

Anti-UACA antibody images

  • ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human ovarian carcinoma tissue section by Immunohistochemistry. Detection: DAB staining
  • ab84478, at a 1/250 dilution, staining UACA in formalin fixed, paraffin embedded human breast carcinoma tissue section by Immunohistochemistry.
    Detection: DAB staining
  • ICC/IF image of ab84478 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84478, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-UACA antibody (ab84478)

ab84478 has not yet been referenced specifically in any publications.

Product Wall

The concentration for this lot of ab84478 0.25 mg/ml.

I hope this is helpful. Please contact us again if you have any further questions.

Thank you for your calls and for your questions. I have heard back from the lab regarding this enquiry.

The blocking peptide was analyzed using HPLC-MSD and has a purity of 90%.We have not used the peptide in an IHC blocking assay at this tim...

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Thank you for your calls and again for your patience.

I have justheard back from my colleague, and the blocking peptide for ab84478 is now available as ab134313-

http://www.abcam.com/UACA-peptide-ab134313.html

Please note ...

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Thank you for your call and for your questions.

I will be in touch with my colleagues to see if we can have this peptide added to the catalog, and I will be back in touch shortly. Please let me know if you have any further questions or if the...

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Thank you for your inquiry. I heard back from the supplying laboratory that they do not determine the precise concentration of IgG for ab84478. This product was prepared to provide specific IHC staining within the recommended range of dilutions.  T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"